Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

江汉盆地钻孔测井资料确定地应力最大水平主压应力方向     

高士钧  储昭坦 《大地测量与地球动力学》1997,(4)

利用钻孔测井资料并运用地层倾角测量信息分析法，给出了江汉盆地地应力最大水平主压应力方向为ＮＥ６０～６５°  相似文献   

SDSS J143030.22-001115.1： A Misclassified Narrow-line Seyfert 1 Galaxy with Flat X-ray Spectrum     

Wei-Hao Bian  Quan-Ling Cui  Li-Hua Chao Department of Physics  Institute of Theoretical Physics  Nanjing Normal University  Nanjing Key Laboratory for Particle Astrophysics  Institute of High Energy Physics  Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2006,6(3):281-286

We used multi-component profiles to model the Hβ and [OⅢ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey (SDSS), Early Data Release (EDR). After subtracting the Hβ contribution from narrow line regions (NLRs), we found that its full width half maximum (FWHM) of broad Hβ line is nearly 2900km s~(-1) , significantly larger than the customarily adopted criterion of 2000 km s~(-1) . With its weak Fe Ⅱ multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLS1s, we should use the Hβ linewidth after subtracting the NLR component.  相似文献   

Multistep matched-field inversion for broad-band data from ASIAEX2001     

Kunde Yang Yuanliang Ma Chao Sun Miller  J.H. Potty  G.R. 《Oceanic Engineering, IEEE Journal of》2004,29(4):964-972

This paper discusses the results of geoacoustic inversion carried out using explosive charge data from the Asian Seas International Acoustic Experiment (ASIAEX) East China Sea (ECS) Experiment. A multifrequency incoherent matched-field inversion processor and a genetic algorithm (GA) are used for the inversion. A multistep matched field inversion approach is presented, which makes use of the varying sensitivities of wave fields at various frequencies to reduce the inversion problem into a sequence of smaller inversions with fewer unknowns to estimate at each stage. Different parameters are estimated using data at different frequencies according to their sensitivities. Inversion results for different areas in the ECS region are summarized and compared with core data.  相似文献   

DEVELOPMENT STRATEGIES OF WATER AND LAND RESOURCES IN THE HEXI REGION,CHINA     

肖洪浪  高前兆  李福兴 《中国地理科学(英文版)》1996,(1)

ＤＥＶＥＬＯＰＭＥＮＴＳＴＲＡＴＥＧＩＥＳＯＦＷＡＴＥＲＡＮＤＬＡＮＤＲＥＳＯＵＲＣＥＳＩＮＴＨＥＨＥＸＩＲＥＧＩＯＮ，ＣＨＩＮＡ肖洪浪，高前兆，李福兴ＤＥＶＥＬＯＰＭＥＮＴＳＴＲＡＴＥＧＩＥＳＯＦＷＡＴＥＲＡＮＤＬＡＮＤＲＥＳＯＵＲＣＥＳＩＮＴＨＥ...  相似文献   

法库地区大型韧性剪切带特征及成因机制     

杨晓波  高恩忆 《国土资源》1996,(2)

通过对辽北法库地区构造岩的观察和研究，确定了本区存在一大型推覆韧性剪切带。根据同构造侵入岩的同位素年龄，确定该韧性剪切带形成时期为海西－印支期，这与天山－兴安褶皱系最终形成时间相一致  相似文献   

A New Solar Radio Spectrometer at 1.10-2.06 GHz and First Observational Results   总被引：1，自引：0，他引：1  

Hui-Rong Ji  Qi-Jun Pu  Yi-Hua Yan  Yu-Ying Liu  Zhi-Jun Chen  Cheng-Ming Tan  Cong-Ling Cheng  De-Bang Lao  Shu-Ke Li  Zhi-Qiang Wang  Min-Hong Yu  Jian-Nong Liu  Li-Kang Zhang  Ji-Yong Gao National Astronomical Observatories  Chinese Academy of Sciences  Beijing  Hebei Semiconductor Research Institute  Shijiazhuang 《中国天文和天体物理学报》2005,5(4):433-441

An improved Solar Radio Spectrometer working at 1.10-2.06 GHz with much improved spectral and temporal resolution, has been accomplished by the National Astronomical Observatories and Hebei Semiconductor Research Institute, based on an old spectrometer at 1-2 GHz. The new spectrometer has a spectral resolution of 4 MHz and a temporal resolution of 5ms, with an instantaneous detectable range from 0.02 to 10 times of the quiet Sun flux. It can measure both left and right circular polarization with an accuracy of 10% in degree of polarization. Some results of preliminary observations that could not be recorded by the old spectrometer at 1-2 GHz are presented.  相似文献   

Silicon limitation on primary production and its destiny in Jiaozhou Bay, China——Ⅳ：Study on cross-bay transect from estuary to ocean   总被引：1，自引：0，他引：1  

Yang Dongfang  Chen Yu  Gao Zhenhui  Zhang Jing  Wang Fan 《中国海洋湖沼学报》2005,23(1):72-90

The authors analyzed the data collected in the Ecological Station Jiaozhou Bay from May 1991 to November 1994, including 12 seasonal investigations, to determine the characteristics, dynamic cycles and variation trends of the silicate in the bay. The results indicated that the rivers around Jiaozhou Bay provided abundant supply of silicate to the bay. The silicate concentration there depended on river flow variation. The horizontal variation of silicate concentration on the transect showed that the silicate concentration decreased with distance from shorelines. The vertical variation of it showed that silicate sank and deposited on the sea bottom by phytoplankton uptake and death, and zooplankton excretion. In this way, silicon would endlessly be transferred from terrestrial sources to the sea bottom. The silicon took up by phytoplankton and by other biogeochemical processes led to insufficient silicon supply for phytoplankton growth. In this paper, a 2D dynamic model of river flow versus silicate concentration was established by which silicate concentrations of 0.028–0.062 μmol/L in seawater was yielded by inputting certain seasonal unit river flows (m³/s), or in other words, the silicate supply rate; and when the unit river flow was set to zero, meaning no river input, the silicate concentrations were between 0.05–0.69 μmol/L in the bay. In terms of the silicate supply rate, Jiaozhou Bay was divided into three parts. The division shows a given river flow could generate several different silicon levels in corresponding regions, so as to the silicon-limitation levels to the phytoplankton in these regions. Another dynamic model of river flow versus primary production was set up by which the phytoplankton primary production of 5.21–15.55 (mgC/m²·d)/(m³/s) were obtained in our case at unit river flow values via silicate concentration or primary production conversion rate. Similarly, the values of primary production of 121.98–195.33 (mgC/m²·d) were achieved at zero unit river flow condition. A primary production conversion rate reflects the sensitivity to silicon depletion so as to different phytoplankton primary production and silicon requirements by different phytoplankton assemblages in different marine areas. In addition, the authors differentiated two equations (Eqs. 1 and 2) in the models to obtain the river flow variation that determines the silicate concentration variation, and in turn, the variation of primary production. These results proved further that nutrient silicon is a limiting factor for phytoplankton growth. This study was funded by NSFC (No. 40036010), and the Director's Fund of the Beihai Sea Monitoring Center, the State Oceanic Administration.  相似文献   

运输系统的区域效应研究   总被引：1，自引：0，他引：1  

高小真 《地理学报》1991,46(1):93-102

运输系统改进—运距缩短—产业布局调整这一空间过程对不同的区域可能具有完全不同的意义。本文通过对一个两区域—两部门经济模型的研究得出结论:当较落后的资源产区与经济中心之间的距离由于运输系统的改进而缩短时,资源产区的经济结构向单一化转变,初级产品的生产和输出在经济建设中逐渐占主导地位,加工工业逐渐萎缩,地方经济发展受到阻碍,而且这种变化是在距离的某一特定值处突然出现的,带有跃变的性质。这一研究结果表明,对区域运输系统的任何改进措施都应该慎而行之,以免造成与本来目标相悖的区域效应。  相似文献   

鄂尔多斯高原晚更新世以来的古冰缘现象及其气候地层学意义   总被引：18，自引：1，他引：17  

董光荣  高尚玉  李保生  吴正 《地理研究》1985,4(1):1-13

本文概述了鄂尔多斯高第三系至全新统地层中融冻(衤习)皱和沙(土)楔两类古冰縁现象的分布与形态特征,探索了它们的成因以及同所在地层和上覆风成沙或黄土的关系,並对古冰縁现象的气候地层学意义作了讨论。  相似文献